METHODOLOGY & COVERAGE · FORM VN-01

Verinome Scientific Methods & Platform Boundaries

A transparent technical disclosure of our evidence grading rubric, provider compatibility matrix, genomic data flow, and clinical limitations.

1.0 · Evidence Classification

The A–D Evidence Grading Rubric

Every genotype-specific finding in Verinome is mapped to a deterministic evidence grade based on clinical consensus, guideline strength, and replication rigor.

A Grade A — Clinical Consensus & High-Penetrance Guidelines

Backed by official clinical consensus guidelines from CPIC (Clinical Pharmacogenetics Implementation Consortium Level 1A/1B) or ACMG (American College of Medical Genetics). Findings have direct pharmacogenomic implications or definitive risk associations replicated across multiple high-powered cohorts.

B Grade B — Replicated Cohorts & Characterized Biochemical Pathways

Supported by multiple independent, peer-reviewed cohort replications or well-characterized enzymatic mechanisms (e.g. CYP1A2 caffeine clearance, ALDH2 acetaldehyde kinetics). Clear statistical significance with moderate effect size.

C Grade C — Preliminary / Single GWAS Associations

Derived from single genome-wide association studies (GWAS) or preliminary observational research. Modest odds ratios; subject to environmental confounders and population-specific portability decay.

D Grade D — Conflicting Literature / Unverified Popular Claims

Widespread popular biohacking claims or candidate gene studies with conflicting replication records (e.g. certain COMT dopamine personality extrapolations). Labeled transparently to prevent consumer deception.

2.0 · Compatibility Matrix

Supported Consumer DNA Formats

Verinome's zero-dependency in-browser parser is tested against raw exports from major direct-to-consumer genotyping providers:

Provider & Chip Version Format Structure Reference Build Compatibility Status
23andMe (v3, v4, v5) 4-Column TSV (rsid, chr, pos, genotype) GRCh37 ✓ Fully Supported (Tested)
AncestryDNA (v1, v2) 5-Column TSV (rsid, chr, pos, allele1, allele2) GRCh37 ✓ Fully Supported (Tested)
MyHeritage DNA Quoted CSV (RSID, CHROMOSOME, POSITION, RESULT) GRCh37 ✓ Fully Supported (Tested)
FamilyTreeDNA (FTDNA) Quoted CSV (RSID, CHROMOSOME, POSITION, RESULT) GRCh37 ✓ Fully Supported (Tested)
Generic TSV / CSV Tab or Comma Delimited with standard rsID headers GRCh37 / GRCh38 ✓ Supported with Fallback
3.0 · Limitations & Boundaries

Genomics Correctness & Array Limitations

Direct-to-consumer DNA tests rely on hybridisation microarrays targeting ~650,000 discrete positions. It is essential to understand what consumer array data can and cannot reveal:

  • Microarray Limitation: Consumer arrays read approximately 0.02% of human single-nucleotide variants. They do not perform whole-genome sequencing (WGS).
  • No Phasing or Copy Number Variations (CNVs): Single-SNP microarrays cannot determine chromosomal phasing (which allele came from which parent) or gene duplications/deletions (e.g. CYP2D6 *5 deletion or *1xN gene duplication).
  • Complex Indels Are Catalog-Only: Complex frameshift deletions (such as BRCA1 185delAG) cannot be called reliably on consumer arrays. Absence from your file does not prove absence in your body.
  • Not a Medical Diagnostic Device: Verinome reports are educational and evidence-graded. Any pharmacogenomic finding or carrier status requires diagnostic clinical confirmation (e.g. Sanger sequencing or targeted qPCR) ordered by a qualified physician.
4.0 · Begin analysis

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